Research Studies
• Acute Toxity Test for Leucozepin
• Acute Toxity Test for Relazovac
• Acute Toxity Test for Apazin
• Leukogenic Studies of Leucozepin
• Sedative Studies of Relazovac
• Appetizing Studies of Apazin
• Clinical Study on Leucozepin
Appetizing Studies of Apazin™
LIFEnhance‘s contract laboratories:
Dr. D. Yang and the Research Team
University of Texas, MD Anderson Cancer Center
Zihong He, Chinese and Western Medicine Institute of Hubei Traditional Chinese Medicine Academy
Abstract:
5g, 2.5g and 1.25g/kg (weight) of Apazin™ , equivalent to 38.46g, 19.23g and 9.62g respectively of crude drugs, which was about 16.44, 8.22, and 4.11 times the clinical dosage for adults, were given (ig) to mice for 12-14 days. The results show that Apazin™ compound increased the food consumption of mice, enhanced the moving distance of activated char powder, the rate of the moving distance, and promoted the phenol red excretion of gastrointestinal tract. Compared with the control group, there was a significant difference. It has been demonstrated that the extract can affect the appetite of mice, have an appetizing effect and improves gastric emptying and the movement of the gastrointestinal tract. However, Apazin™ did not show an obvious effect on the weight of the mice.
Materials:
1. Apazin™ extract, was provided by LIFEnhance, Inc., with batch number 20020401. 0.13g of extract was equivalent to 1g of crude drug. It was stored in refrigerator at 4ºC. When used, it was compounded with distilled water into the needed concentration.
2. Janwei Xiaoshi Pian (a digestive medicine), was provided by Jianzhong Medicine Industry Joint Stock Co. Ltd, with batch number 0203064.
Reagent: Analytically Pure
Instrument: 721 spectrophotometer
3. Animals, provided by Hubei Animal Experimental center, were Kunming-breed mice with the weight of 18--20±1g and with the same number of male and female. The certificate of inspection was 10-082.The mouse food was purchased from the food factory of Hubei Animal Experimental Center.
Methods:
1. The Effect on the Appetite of mice:
According to the literature[1], 60 mice, with an equal number of male and female, and a weight of 19±1g, were divided into five groups at random: the control group, Jianwei Xiaoshi Pian group and groups of Apazin™ for large, medium and small dosage. The mice were respectively given (ig) Ns 20ml/kg, 2.4g of Jianwei Xiaoshi Pian, and 5g, 2.5g and 1.25g of Apazin™, once daily for 14 days. The mice were raised in different cages, the food was weighed after drying, and food was given to the mice. The left-over food was weighed again to accurately record the food consumption of mice. The same steps were repeated daily. In this way, the food consumption of various groups of mice and their weights were recorded against that of the control group during the same period. It can be seen that the Jianwei Xiaoshi Pian group and the three groups of Apazin™ can increase the food consumption of mice, proving the effect on the appetite of mice. There is a significant difference compared with the control group, but there is no obvious effect on the weight of the mice.
2. The Effect on the Movement of Small Intestine
According to the literature[2], the mice were divided into groups in the same way as above. After they were given (ig) the Apazin™ extract for 12 consecutive days, they received no food for a 24 hour period. As a sign substance, activated char powder was compounded into 10? suspension with normal saline solution. The medicinal group was given (ig) medicine instead of normal saline solution, 0.2ml for per 10g of weight. After 20 minutes, the mice died due to dislocation of the cervical vertebra to remove the stomach and intestine at once. They were put on a glass board to measure the moving distance of activated char powder in the intestine and the whole length of the small intestine (from pylorus to ileocecum), and the percentage of the movement was calculated.
3. The Quantitative Measurement of Phenol Red
According to the literature[3], 50 Kunming-breed mice with the same number of male and female were divided into five groups, given(ig) respectively NS, Jianwei Xiaoshi Pian (2.4g/kg) and 6g,3g,1.5g/kg of Apazin™. 0.2ml was given (ig) per 10g of weight, once daily for 12 days. After receiving no food for 36 hours, the mice were given 0.01% of phenol red paste (i.g) 0.2ml per 10g of weight. 20 minutes later, the mice were decapitated to remove and clean the small intestine. The optical density was measured at the wave length of 560 nm of 721 spectrophotometer.
Results:
The results show that 5g and 2.5g dosage of Apazin™ can obviously increase the moving distance and the percentage of char powder, there is a significant difference compared with the control group. It is proven that the Apazin™ compound can increase the gastrointestinal peristalsis, hence having appetizing effect.
References:
[1][3]Li Yikui .Experimental Methodology of Pharmacology of Traditional Chinese Medicine [m] Shanghai: Shanghai Science and Technology Press ,1999:149,321
[2]Chen Qi. Research Methodology of Pharmacology of Traditional Chinese Medicine[M] Beijing: People’s Health Press,1993:335
|
 |
|
