Research Studies
• Acute Toxity Test for Leucozepin
• Acute Toxity Test for Relazovac
• Acute Toxity Test for Apazin
• Leukogenic Studies of Leucozepin
• Sedative Studies of Relazovac
• Appetizing Studies of Apazin
• Clinical Study on Leucozepin
Leukogenic Studies of Leucozepin™
LIFEnhance‘s contract laboratories:
Dr. D. Yang and the Research Team
University of Texas, MD Anderson Cancer Center
Zihong He, Chinese and Western Medicine Institute of Hubei Traditional Chinese Medicine Academy
Abstract:
The subjects of the experiment are normal mice and S180-bearing mice. Mice leukopenia models were prepared by iP cyclophosphamide to observe the effects of Leucozepin™ on the peripheral leukocyte count, bone marrow karyocyte count and tumor weight of mice models. The results show that 8g/kg and 4g/kg of Leucozepin™ can obviously increase the counts of white blood cell and bone marrow karyocyte of the leukopenia model and does not affect the inhibitive effect of cyclophosphamide on tumors.
Materials:
1. Mice, Kunming-breed mice, half of whom are male, with the weight of 18-22g, were provided by the Animal Experiment center of Hubei Medical Academy. The certificate of inspection is 19-082.
2. Tumor cells, sarcoma 180 (S180) of mice, was provided by the Tumor Research Section of the Pharmacy college of Tongji Medical University.
3. Leucozepin™ provided by LIFEnhance, Inc., is pyknotic extract. 1g crude drug is equal to 0.11g extract. It was mixed with distilled water into the needed concentration.
4. Cyclophosphamide (CTX), 200mg per bottle, is made by Jiang Su Heng Rui Medicine Limited Company and the batch number is 02022821.
5. Leucogen (a medicine that increases white blood cells) is made by Shanghai Jinshan Medicine-making Limited Company and the batch number is 000502.
6. Tabellae Batiloli is produced by Jiang Su PengYao Pharmacy Limited Company and the batch number is 000316-2.
Methods:
1. Effect of Leucozepin™ on cyclophosphamide induced leukopenia in mice:
84 mice, according to sex and weight, were divided into 6 groups at random: normal control group, model control group, large dosage group(8g/kg), medium dosage group(4g/kg), small dosage group(2g/kg), and Tabellae Batiloli positive group (40mg/kg). Before the model was prepared, the mice were given the extract (ig) for 6 days (the normal control group and the model control group were given the same amount of distilled water). From the seventh day iP cyclophosphamide was given to the five groups - excluding the normal control group - once daily for two days. From the day when the model was prepared, they were given Leucozepin™ for six consecutive days. On the first and the fourth day after the model was prepared, the tails of the mice were cut to take a blood sample to check the peripheral leukocyte count. After the last blood sample was taken, the mice died due to dislocation of cervical vertebra. The right femur was removed according to the literature[1] to count the bone marrow karyocyte and t-check was carried out among groups.
2. Effect of Leucozepin™ on cyclophosphamide induced leukopenia in S180-bearing mice:
According to the literature [2], S180 cells were inoculated under the skin of the right armpit of 72 mice, half of whom were male (0.2ml each). The next day, the mice were divided into 6 groups at random, S180-bearing control group, (20ml/kg distilled water), CTX model group (20ml/kg distilled water), large dosage group, medium dosage group, small dosage group (i.e. 8, 4, 2g/kg) and Leucogen positive group (20mg/kg). The next day after the inoculation they were given the medicine (ig) or distilled water, once daily for 12 consecutive days. From the 7th day, ip cyclophosphamide 100mg/kg was given to the five groups – excluding the S180-bearing control group – once daily for two days. On the first and the fourth day after the model was prepared, the tails of the mice were cut to take a blood sample to check the peripheral leukocyte count. After the last blood sample was taken, the mice died due to dislocation of cervical vertebra. The mice were dissected and the tumors were removed and weighed. According to the literature [2], the inhibitive rate of the tumors was calculated. Simultaneously, the right femur was removed to count the bone marrow karyocyte and t-check was carried out among the groups.
Results:
The effect of Leucozepin™ on cyclophosphamide induced leukopenia and the decrease of bone marrow karyocyte count in normal mice. The results reveal that ip cyclophosphosphamide could induce leukopenia and decrease bone marrow karyocyte count, which is obviously different from that of the control group. 8g/kg and 4g/kg of Leucozepin™ and Tabellae Batiloli can obviously increase the counts of white blood cell and bone marrow karyocyte as compared to the results of the model control group.
|
 |
|
